Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide
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چکیده
منابع مشابه
Separation of Thyroidal Iodoproteins and Purification of Thyroglobulin by Gel Filtration and Density Gradient Centrifugation.
Thyroglobulin, the major protein component of normal thyroid glands, contains more than 80% of the thyroidal iodine and has been considered the only source of thyroid hormones (1). Aqueous thyroid extracts contain, besides thyroglobulin, other proteins in variable amounts. Essentially, four methods have been used for the characterization of the various components in such extracts and for the pu...
متن کاملDensity gradient centrifugation of rubella virus.
Rubella virus was centrifuged in sucrose density gradients. One of two densities could be ascribed to the virus, depending upon the suspending medium used. The virus was found at a density of 1.16 g/cm(3) after centrifugation for 18 hr in sucrose gradients prepared in distilled water. By contrast, when the sucrose gradients were prepared in tris(hydroxymethyl)aminomethane (Tris)buffer containin...
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The solid-phase radioimmunoassay (RIA) method developed in our laboratory for demonstrating rubella virus-specific immunoglobulin G (IgG) antibodies (Kalimo et al., 1976) was further developed for demonstrating IgM antibodies. A total of 188 serum specimens were tested. The statistical probability of obtaining a false-positive IgM result, based on determinations of 100 rubella-negative sera, wa...
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Fractionation of fully sporulated cultures of Bacillus thuringiensis by density gradient centrifugation in NaBr produced two bands which were identified as poly-beta-hydroxybutyrate. This technique generated high yields of membrane-bound and unbound granules of exceptional purity and degree of polymerization.
متن کاملGel-filtration and Density-gradient-centrifugation Studies of the Molecular Weights of Halobacterium Cutirubrum
1. Conditions have been established for the estimation of molecular weights of proteins by analytical gel filtration and sucrose-density-gradient centrifugation in 2.5M-potassium chloride-l M-sodium chloride; Halobacterium cutirubrum polynucleotide phosphorylase, DNA-dependent RNA polymerase and RNA-dependent RNA polymerase have been studied by these methods. 2. The RNA-dependent polymerase has...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 1981
ISSN: 0166-0934
DOI: 10.1016/0166-0934(81)90032-x